Abstract:Malaria is a life-threatening disease caused by parasites transmitted to people and animals through the bites of infected mosquitoes. We biosynthesized silver nanoparticles (AgNP) using Aristolochia indica extract as reducing and stabilizing agent. AgNP were characterized by UV–vis spectroscopy, FTIR, SEM, EDX and XRD. In laboratory, LC50 of A. indica extract against Anopheles stephensi ranged from 262.66 (larvae I) to 565.02 ppm (pupae). LC50 of AgNP against A. stephensi ranged from 3.94 (larvae I) to 15.65 ppm (pupae). In the field, the application of A. indica extract and AgNP (10 × LC50) leads to 100% larval reduction after 72 h. In laboratory, 24-h predation efficiency of Diplonychus indicus against A. stephensi larvae was 33% (larvae II) and 57% (larvae III). In AgNP-contaminated environment (1 ppm), it was 45.5% (larvae II) and 71.75% (larvae III). Overall, A. indica-synthesized AgNP may be considered as newer and safer control tools against Anopheles vectors.