English  |  正體中文  |  简体中文  |  Items with full text/Total items : 26988/38789
Visitors : 2350374      Online Users : 35
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/44644

Title: Short- and long-term cryopreservation of sperm and sperm suspension of the grouper, Epinephelus malabaricus (Bloch and Schneider)
Authors: Chao, N.H.;Tsai, H.P.;Liao, I C.
Date: 1992
Issue Date: 2017-11-24T02:44:18Z
Publisher: Asian Fisheries Science
Abstract: Abstract:
Essential factors which affect the feasibility of cryopreserving sperm of the grouper, Epinephelus malabaricus (Bloch and Schneider), were determined by comparing percentage of motile spermatozoa, duration of storage life and fertilization rate of eggs when available. Fertility of sperm after thawing was maintained by using 10% dimethyl sulfoxide (DMSO) as a cryoprotectant with less than 10 minutes equilibration. An optimal ratio of 85% for sperm suspension from sliced testis or fresh milt diluted 20 times in Ringer solution for marine fish: 10% DMSO: 5% glucose is suggested. The addition of 15% Menezo Medium B2 INRA to the milt mixture resulted in the retention of higher progressive motility of cryopreserved sperm. The use of isopropanol with dry ice at -75°C or liquid nitrogen vapor at -100°C as freezing medium simplified the freezing procedure. Analysis of the motility of post-thawing sperm using the Hamilton-Thorn motility analyzer showed that the performance of one-and three-month old cryopreserved grouper spermatozoa was slightly poorer in terms of path velocity, progressive velocity, track speed and straightness. The use of microwaves in thawing frozen milt in polyethylene straws was found feasible and convenient with an optimum degree of power at 40% and conditional duration of 30-70 seconds. The post-thawing fertility of the spermatozoa was better when frozen immediately upon collection or right after transporting the material of raw milt or sperm suspension at 4°C for 1-2 hours. Fertility records of 78±18%, 83±3%, 95±0%, 75±32%, 13±1%, 95±0% and 66±12% were obtained for 1-, 17-, 41-, 54-, 128-, 278- and 291-day cryopreserved sperm (65.58-91.92% in controls) during the spawning seasons of 1990 and 1991.
Preliminary extension to a private hatchery in 1991 resulted in additional production of about 50 million fertilized eggs by using cryopreserved sperm of E. malabaricus eight times when fresh milt was not available in the field.
Relation: 5(1), pp.103-116
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/44644
Appears in Collections:[廖一久院士專區] 期刊論文

Files in This Item:

There are no files associated with this item.



All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback