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|Title: ||A fast-growing trait of loach (Misgurnus anguillicaudatus) obtained by sperm-mediated gene transfer biotechnology|
|Authors: ||Tsai, H.J.;Tseng, F.S.;Liao, I C|
|Issue Date: ||2017-11-23T01:25:16Z
|Publisher: ||AFS Special Publication No.10|
An antifreeze protein gene promoter from ocean pout Macrozoarces americanus linked to a chinook salmon Oncorhynchus tschawytscha growth hormone cDNA (opAFPGHc) was introduced into loach Misgurnus anguillicaudatus sperm via electroporation. Compared to the untreated sperm, the fertilization rate driven by the electroporated-sperm decreased slightly, but had not significant effect on hatchability. The success rate of gene transfer was 50%. Genomic Southern blotting demonstrated that DNA from transgenic loach contained positive band(s) with higher (37.5%), the same, or lower molecular size than the transferred opAFPGHc fragment. There was a mosaic distribution of exogenous DNA in a wide variety of tissues analyzed. The average weight gain between the 14th and 161th day of the experimental group was 2.5-fold higher than that of the control and the mock-treated groups. The body length distribution of the experimental group was rather widely scattered instead of being normally distributed.
|Relation: ||pp. 75-93|
|Appears in Collections:||[廖一久院士專區] 會議論文|
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