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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43582

Title: Production of Bacillus subtilis-fermented red alga Porphyra dentata suspension with fibrinolytic and immune-enhancing activities
Authors: Hong-Ting Victor Lin;Pai-An Hwang;Tzu-Chun Lin;Guo-Jane Tsai
Contributors: 國立臺灣海洋大學:生命科學系
Keywords: Bacillus subtilis;Porphyra dentata;fermentation;fibrinolytic activity;immune-enhancing activity
Date: 2014-06
Issue Date: 2017-10-12T06:18:44Z
Publisher: Biosci Biotechnol Biochem
Abstract: Abstract:The fermented marine alga Porphyra dentata suspension was tested for its fibrinolytic and immune-enhancing activities. An isolated Bacillus subtilis N2 strain was selected for its fibrinolytic activity on fibrin plates. After investigating the effects of biomass amounts of P. dentata powder in water, various additives including sugars, nitrogen-containing substances, lipids and minerals, and cultural conditions of temperature and agitation in flask, the highest fibrinolytic activity in the cultural filtrate was obtained by cultivating N2 strain in 3% (w/v) P. dentata powder suspension containing 1% peanut oil at 37 °C, 150 rpm for 48 h. A fermentor system was further established using the same medium with controlled pH value of 7.0 at 37 °C, 150 rpm, 2.0 vvm for 48 h for the best fibrinolytic activity. The fermented product also showed its immune-enhancing activity by increasing cell proliferation and stimulating the secretion of IL-1β, IL-6, and TNF-α in J774.1 cells.
Relation: 78(6), pp.1074-1081
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43582
Appears in Collections:[生命科學系] 期刊論文

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