English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27228/39071
Visitors : 2414000      Online Users : 69
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43566

Title: Using Copper Ions to Amplify ROS-Mediated Fluorescence for Continuous Online Monitoring of Extracellular Glucose in Living Rat Brain
Authors: Su CK Chen CY Tseng PJ Sun YC
Contributors: 國立臺灣海洋大學:生命科學系
Keywords: Extracellular fluid;GlucoseIn vivo;Online monitoring;Reactive oxygen species
Date: 2015-02
Issue Date: 2017-09-22T03:17:29Z
Publisher: Biosensors and Bioelectronics
Abstract: Abstract:In this study we developed a facile and sensitive method for continuous monitoring of extracellular glucose concentration in living rat brain through microdialysis (MD) sampling in conjunction with (i) online sample derivatization using glucose oxidase to generate H2O2, which converted a reactive oxygen species-responsive fluorescent dye, 2′,7′-dichlorodihydrofluorescein (DCFH), into fluorescent species, and (ii) a novel non-immobilized enzyme-based fluorescence assay strategy, featuring copper ion (Cu2+)-facilitated amplification of the fluorescence intensity. After evaluating the experimental conditions for glucose oxidation and fluorescence generation, the introduction of Cu2+ ions to this system resulted in an additional 51-fold amplification of the net fluorescence intensity. By sequentially loading brain microdialysate into the dual sample collection loops, the sampling frequency was 7.5 h−1. Based on a 40-μL sample volume, the system's detection limit reached as low as 0.18 mM, sufficiently accurate to determine the extracellular glucose concentrations in living rat brains. To demonstrate the proposed system's practical performance and applicability, we conducted (i) spike analyses of biomolecule-rich fetal bovine serum sample, confirming that the analytical reliability was similar to that of a commercial glucose kit, and (ii) in vivo dynamic monitoring of the extracellular glucose concentrations in living rat brains after inducing neural depolarization by perfusing a high-K+ medium from the MD probe.
Relation: 64(15), pp.535-541
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43566
Appears in Collections:[生命科學系] 期刊論文

Files in This Item:

File Description SizeFormat

All items in NTOUR are protected by copyright, with all rights reserved.


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback