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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43564

Title: Online Open-Tubular Fractionation Scheme Coupled with Push–Pull Perfusion Sampling for Profiling Extravasation of Gold Nanoparticles in a Mouse Tumor Model
Authors: Cheng-Kuan Su;Po-Jen Tseng;Meng-Han Lin;Hsien-Ting Chiu;Andrea del Vall;Yu-Fen Huang;Yuh-Chang Sun
Contributors: 國立臺灣海洋大學:生命科學系
Keywords: Extravasation;Fractionation;Gold nanoparticle;Push–pull perfusion;Tumor extracellular fluid
Date: 2015-07-10
Issue Date: 2017-09-22T02:51:22Z
Publisher: Journal of Chromatography A
Abstract: Abstract:The extravasation of administered nano-drug carriers is a critical process for determining their distributions in target and non-target organs, as well as their pharmaceutical efficacies and side effects. To evaluate the extravasation behavior of gold nanoparticles (AuNPs), currently the most popular drug delivery system, in a mouse tumor model, in this study we employed push–pull perfusion (PPP) as a means of continuously sampling tumor extracellular AuNPs. To facilitate quantification of the extravasated AuNPs through inductively coupled plasma mass spectrometry, we also developed a novel online open-tubular fractionation scheme to allow interference-free determination of the sampled extracellular AuNPs from the coexisting biological matrix. After optimizing the flow-through volume and flow rate of this proposed fractionation scheme, we found that (i) the system's temporal resolution was 7.5 h−1, (ii) the stability presented by the coefficient of variation was less than 10% (6-h continuous measurement), and (iii) the detection limits for the administered AuNPs were in the range 0.057–0.068 μg L−1. Following an intravenous dosage of AuNPs (0.3 mg kg−1 body weight), in vivo acquired profiles indicated that the pegylated AuNPs (PEG-AuNPs) had greater tendency toward extravasating into the tumor extracellular space. We also observed that the accumulation of nanoparticles in the whole tumor tissues was higher for PEG-AuNPs than for non-pegylated ones. Overall, pegylation appears to promote the extravasation and accumulation of AuNPs for nano-drug delivery applications.
Relation: 7402, pp.1-7
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43564
Appears in Collections:[Department of Life Science] Periodical Articles

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