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题名: Genetic characterization and in vivo imaging analysis of novel zebrafish pigment mutants
作者: Hsu CC;Pai WY;Lai CY;Lu MW;Her GM
贡献者: 國立臺灣海洋大學:生命科學系
日期: 2013-04-12
上传时间: 2017-09-15T02:47:42Z
出版者: Journal of Fish Biology
摘要: Abstract:This study reports the isolation and characterization of a new type of transparent zebrafish Danio rerio mutant called pinky (pk), which has been visually isolated from a spontaneous mutation in a D. rerio colony. The pk larvae possess complex mutations affecting pigmentation because of missing pigment cells or a dramatic reduction in the chromatophore number. The pk displays a totally colourless phenotype and adult body transplant with no other obvious external morphological abnormalities, except for a red retina. The molecular analysis results in several candidate genes, hps1, ap3m2 and rabggta, implicated in the Hermansky–Pudlak syndrome (HPS) genes associated with HPS in pk. To demonstrate its applications of deep-tissue imaging, this study examines green fluorescent protein alone or with other fluorescent proteins to investigate their capability for using multilabelling purposes in live adult pk. In this study, pk is particularly valuable for tissue cell labelling and internal organogenesis studies because of its optical clarity in the adult body.
關聯: 82(5), pp.1671-1683
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43543
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