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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43535

Title: Comparative effects of mercury (ll) and cadmium on Muts homolog 6(Msh6)-mediate DNA mismatch binding activities in zebrafish (Danio rerio) embryo
Authors: Tsung-Nan Ho;Shih-Tsung Sung;Kuan-Ming Huang;Todd Hsu
Contributors: 國立臺灣海洋大學:生命科學系
Date: 2015-07
Issue Date: 2017-09-14T06:38:50Z
Publisher: Journal of Biochemical and Molecular Toxicology
Abstract: Abstract:MutS homolog 6 (MSH6) of the MSH2–MSH6 complex binds simple mispairs and small insertion-deletion loops (IDL) then initiates DNA mismatch repair in eukaryotes. We have shown the ability of Cd2+ to downregulate msh2/msh6 expression in zebrafish embryos via oxidative stress. This study explored the effects of Cd2+ and Hg2+ on MSH6-mediated mismatch binding activities. MSH6-mediated G-T and IDL-specific binding activities were significantly inhibited at similar levels after exposing zebrafish embryos at 1 h postfertilization) to HgCl2 or CdCl2 at 1.0 to 2.5 μM for 9 h, but MSH6 synthesis was found to be less sensitive to Hg2+ than to Cd2+. Real-time RT-PCR and in situ hybridization also detected a weaker susceptibility of MSH gene transcription to Hg2+. The weaker response of MSH gene activities to Hg2+ correlated with the lower oxidative stress-inducing potential of Hg2+. Hence, Hg2+ targets mismatch sensing capacity at protein function rather than at transcription level.
Relation: 29(11), pp.513-520
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43535
Appears in Collections:[生命科學系] 期刊論文

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