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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43522

Title: Cryopreservation of the gorgonian endosymbiont Symbiodinium
Authors: Gabriella Chong;Sujune Tsai;Li-Hsueh Wang;Chih-Yang Huang;Chiahsin Lin
Contributors: 國立臺灣海洋大學:水產養殖學系
Date: 2016-01
Issue Date: 2017-09-06T03:06:40Z
Publisher: Scientific Reports
Abstract: Abstract:The study focused on finding a suitable cryoprotectant (CPA) and an optimum freezing protocol for the cryopreservation of the endosymbiotic dinoflagellates (Symbiodinium, clade G) of Junceella fragilis wherein the success of experiments is crucial to both scientific and ecology studies. A two-step freezing technique was developed. The viability of the thawed dinoflagellates was assayed using the adenosine triphosphate (ATP) bioassay for the first time and was further confirmed through the culturing of dinoflagellates in vitro. The results suggested that 30 min was the most suitable holding time for the dinoflagellates, and the samples produced highest viability when suspended at 5 cm from the surface of LN2. Results also showed that 1 M methanol with 0.4 M sucrose was the most effective CPA, yielding the highest viability (56.93%). Although cell densities of both cryopreserved and control group suffered an initial decline of culture, the cell densities were maintained throughout the remaining duration. In the present study, the cryopreservation of clade G endosymbiont algae was studied for the first time and the method described here could be applied for future studies on symbiotic algae cryopreservation.
Relation: 6(18816), pp.1-9
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43522
Appears in Collections:[水產養殖學系] 期刊論文

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