National Taiwan Ocean University Institutional Repository:Item 987654321/43491
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题名: Isolation of a novel polyomavirus, related to Japanese eel endothelial cell-infecting virus, from marbled eels, Anguilla marmorata
作者: Chiu-Ming Wen;M M Chen;Chun Shun Wang;P C Liu;Fan-Hua Nan
贡献者: 國立臺灣海洋大學:水產養殖學系
关键词: Anguilla;T-antigen;eel diseases;haemorrhage;polyomavirus;viral endothelial cell necrosis of eel
日期: 2015-11
上传时间: 2017-08-29T02:40:43Z
出版者: Journal of Fish Diseases
摘要: Abstract:Marbled eels, Anguilla marmorata (Quoy & Gaimard), cultured in Taiwan exhibited haemorrhage and mortality in January 2012. The severely diseased eels bled from the gills and showed congestion of the central venous sinus of the gill filaments and haemorrhage throughout the body similar to viral endothelial cell necrosis of eel. In this study, a novel polyomavirus (AmPyV) was isolated from the diseased eels using the AMPF cell line established from the pectoral fin of healthy marbled eels. AmPyV was found to encode a long T-antigen orthologous gene. Phylogenetic analysis showed that AmPyV was closely related to Japanese eel endothelial cell-infecting virus. PCR assays revealed AmPyV infection throughout the systemic organs. AmPyV proliferated in the AMPF, EK-1 and EO-2 cells at temperatures 25-30 °C, and the progeny virus yields were 10(7.0) , 10(7.4) and 10(7.7) TCID50 mL(-1) , respectively. The purified virions were icosahedral particles, 70-80 nm in diameter. No clinical signs or mortality was observed among the eels injected with the virus; however, the virus was reisolated from the brain, eyes, kidneys, fins and gills of infected eels 2 month after injection. Our results suggest that AmPyV exhibits a latent infection. Pathogen of the disease needs to study further.
關聯: 39(7):889-97
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43491
显示于类别:[水產養殖學系] 期刊論文

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