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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43476

Title: SNP detection by parallel targeted sequencing from degraded DNA samples in Haliotis diversicolor
Authors: Peng Ren;Ya wei Shen;Ze kun Huang;Miao qin Huang;Xuan Luo;Jin-Chywan Gwo;Cai huan Ke;Wei wei You
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: Haliotis diversicolor;Degraded DNA;Single nucleotide polymorphisms;Parallel target sequencing
Date: 2016-11
Issue Date: 2017-08-17T07:25:48Z
Publisher: Conservation Genetics Resources
Abstract: Abstract:The small abalone, Haliotis diversicolor, is an economically important shellfish species in East Asia. The wild stocks have been highly threatened due to overfishing in the past several decades. Taking into account conservation and recovery of wild resources, long preserved samples were used to analyze population structure. However, extreme fragmentation of DNA limited the use of these degraded DNA samples. In this study, a total of 96 novel single nucleotide polymorphisms (SNPs) were identified based on transcriptomes of H. diversicolor. The parallel target sequencing was then used to examine one wild population collected in 2003 from Sanya Bay, Hainan Province with degraded DNA. Nineteen loci appeared in more than 80% of individuals and were polymorphic. This study provides a valuable method for using degraded DNA and molecular markers for genetic diversity analysis of H. diversicolor.
Relation: 9(2), pp.193-195
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43476
Appears in Collections:[水產養殖學系] 期刊論文

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