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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43474

Title: AFLP-SSCP: A Useful AFLP-Based Method for Informative SNPs Discovery in Non-Model Organisms
Authors: Te-Hua Hsu;Yue Ning;Jin-Chywan Gwo
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: AFLP;SNPs;Molecular Marker;SSCP
Date: 2014-06
Issue Date: 2017-08-17T03:23:33Z
Publisher: Advances in Biological Chemistry
Abstract: Abstract:Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation among individuals of a species. Recently, in spite of the development of high-throughput genotyping technologies, SNPs have been applied as markers for population genetic and high-density genetic mapping. However, the high costs of SNPs discovery and genotyping assay limit the applications of SNP markers in non-model organisms. In this study, we present a cheap and convenient AFLP-based (Amplified fragment length polymorphism) strategy that is highly efficient for developing informative SNP markers without any prior information. We developed SNP markers in a non-model and economic aquaculture species Asian Seabass (Lates calcarifer), and discussed the potential use of the combinations of AFLP and AFLP-SSCP.
Relation: 4, pp.376-381
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43474
Appears in Collections:[水產養殖學系] 期刊論文

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