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http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43474
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| Title: | AFLP-SSCP: A Useful AFLP-Based Method for Informative SNPs Discovery in Non-Model Organisms |
| Authors: | Te-Hua Hsu;Yue Ning;Jin-Chywan Gwo |
| Contributors: | 國立臺灣海洋大學:水產養殖學系 |
| Keywords: | AFLP;SNPs;Molecular Marker;SSCP |
| Date: | 2014-06
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| Issue Date: | 2017-08-17T03:23:33Z
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| Publisher: | Advances in Biological Chemistry |
| Abstract: | Abstract:Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation among individuals of a species. Recently, in spite of the development of high-throughput genotyping technologies, SNPs have been applied as markers for population genetic and high-density genetic mapping. However, the high costs of SNPs discovery and genotyping assay limit the applications of SNP markers in non-model organisms. In this study, we present a cheap and convenient AFLP-based (Amplified fragment length polymorphism) strategy that is highly efficient for developing informative SNP markers without any prior information. We developed SNP markers in a non-model and economic aquaculture species Asian Seabass (Lates calcarifer), and discussed the potential use of the combinations of AFLP and AFLP-SSCP. |
| Relation: | 4, pp.376-381 |
| URI: | http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43474 |
| Appears in Collections: | [水產養殖學系] 期刊論文
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