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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43470

Title: Rapid sex identification of papaya (Carica papaya) using multiplex loop-mediated isothermal amplification (mLAMP)
Authors: Hsu, T.-H.;Gwo, J.-C.;Lin, K.-H.
Contributors: 國立臺灣海洋大學:水產養殖學系
Date: 2012-10
Issue Date: 2017-08-17T02:56:08Z
Publisher: Planta
Abstract: Abstract:Papaya (Carica papaya L.) is established as a cash crop throughout the tropical and subtropical regions due to its easy adaptation to diverse agricultural conditions, high yields, and prompt returns. The sex types of papaya plants are hermaphrodite, male, and female. Among them, hermaphroditic plants are the major type in papaya production, because the fruit has commercial advantages over that of the other sexes. Sex inheritance in papaya is determined by the M and M(h) dominant alleles in males and hermaphrodites, respectively, and a recessive m allele in females. Currently, all hermaphrodite seeds are not available due to the lethality of dominant homozygosity. Therefore, in this study, six male-hermaphrodite-specific markers were developed for a rapid sex identification using multiplex loop-mediated isothermal amplification (mLAMP) to efficiently and precisely select hermaphroditic individuals in the seedling or early growth stage. The LM1-LAMP assay consisted of two sex-LAMP reactions for amplifying two male-specific markers (T12 and Cpsm90) in one reaction, and showed several advantages in terms of a rapid reaction time (<1 h), isothermal conditions (less equipment required), a high efficiency (0.5 ng of DNA required in the reaction mixture), and an economical reaction system (5 μl in volume). The established method can be easily performed in the field by visual inspection and facilitates the selection of all hermaphroditic individuals in papaya production.
Relation: 236(4), pp.1239-1246
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43470
Appears in Collections:[水產養殖學系] 期刊論文

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