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Title: Identification and characterization of a late gene encoded by grouper iridovirus 2L (GIV-2L)
Authors: H-Y Lin, C-F Cheng
P P Chiou
C-J Liou
J-C Yiu
Y-S Lai
Contributors: 國立臺灣海洋大學:水產養殖學系
Date: 2014-10
Issue Date: 2017-08-15T07:47:19Z
Publisher: Journal of Fish Disease
Abstract: Abstract:Grouper iridovirus (GIV) belongs to the Ranavirus genus and is one of the most important viral pathogens in grouper, particularly at the fry and fingerling stages. In this study, we identified and characterized the GIV-2L gene, which encodes a protein of unknown function. GIV-2L is 1242 bp in length, with a predicted protein mass of 46.2 kDa. It displayed significant identity only with members of the Ranavirus and Iridovirus genera. We produced mouse monoclonal antibodies against the GIV-2L protein by immunizing mice with GIV-2L-His-tag recombinant protein. By inhibiting de novo protein and DNA synthesis in GIV-infected cells, we showed that GIV-2L was a late gene during the viral replication. Finally, immunofluorescence microscopy revealed that GIV-2L protein accumulated in both the nucleus and cytoplasm of infected cells. These results offer important insights into the pathogenesis of GIV.
Relation: 38(10), pp.881-890
Appears in Collections:[Department of Aquaculture] Periodical Articles

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