National Taiwan Ocean University Institutional Repository:Item 987654321/43457
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 28607/40644
Visitors : 4692865      Online Users : 315
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43457

Title: Transgenic Expression of Omega-3 PUFA Synthesis Genes Improves Zebrafish Survival during Vibrio vulnificus Infection
Authors: Chih-Lun Cheng;Shin-Jie Huang;Chih-Lu Wu;Hong-Yi Gong;Chuian-Fu Ken;Shao-Yang Hu;Jen-Leih Wu
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: Omega-3 PUFAs;Anti-inflammation;Anti-bacteria;Vibrio vulnificus;Transgenesis
Date: 2015-11-17
Issue Date: 2017-08-14T01:58:26Z
Publisher: Journal of Biomedical Science
Abstract: Abstract:
Background
Highly desaturated n-3 polyunsaturated fatty acids (PUFAs), such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are synthesized by desaturases and elongase. They exert hepatoprotective effects to prevent alcoholic fatty liver syndrome or cholestatic liver injury. However, it is unclear how n-3 PUFAs improve immune function in liver. Vibrio vulnificus, a gram-negative bacterial pathogen, causes high mortality of aquaculture fishes upon infection. Humans can become infected with V. vulnificus through open wounds or by eating raw seafood, and such infections may result in systemic septicemia. Moreover, patients with liver diseases are vulnerable to infection, and are more likely than healthy persons to present with liver inflammation following infection. This study quantified n-3 PUFAs and their anti-bacterial effects in Fadsd6 and Elvol5a transgenic zebrafish.

Results
Two transgenic zebrafish strains with strong liver specific expression of Fadsd6 and Elvol5a (driven by the zebrafish Fabp10 promoter) were established using the Tol2 system. Synthesis of n-3 PUFAs in these strains were increased by 2.5-fold as compared to wild type (Wt) fish. The survival rate in 24 h following challenge with V. vulnificus was 20 % in Wt, but 70 % in the transgenic strains. In addition, the bacteria counts in transgenic fish strains were significantly decreased. The expression levels of pro-inflammatory genes, such as TNF-α, IL-1β, and NF-κB, were suppressed between 9 and 12 h after challenge. This study confirms the anti-bacterial function of n-3 PUFAs in a transgenic zebrafish model.

Conclusions
Fadsd6 and Elvol5a transgenic zebrafish are more resistant to V. vulnificus infection, and enhance survival by diminishing the attendant inflammatory response.
Relation: 22, pp.103
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43457
Appears in Collections:[Department of Aquaculture] Periodical Articles

Files in This Item:

File Description SizeFormat
index.html0KbHTML63View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback