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题名: Interferon regulatory factor-1 (IRF-1) is involved in the induction of phosphatidylserine receptor (PSR) in response to dsRNA virus infection and contributes to apoptotic cell clearance
作者: Hsin-Chia Kung;Øystein Evensen;Jiann-Ruey Hong;Chia-Yu Kuo;Chun-Hsi Tso;Fang-Huar Ngou;Ming-Wei Lu;Jen-Leih Wu
贡献者: 國立臺灣海洋大學:水產養殖學系
关键词: phosphatidylserine receptor (PSR);interferon regulatory factor-1 (IRF-1);interferon (IFN);apoptosis
日期: 2014-10-23
上传时间: 2017-08-10T03:52:30Z
出版者: International Journal of Molecular Sciences
摘要: Abstract:The phosphatidylserine receptor (PSR) recognizes a surface marker on apoptotic cells and initiates engulfment. This receptor is important for effective apoptotic cell clearance and maintains normal tissue homeostasis and regulation of the immune response. However, the regulation of PSR expression remains poorly understood. In this study, we determined that interferon regulatory factor-1 (IRF-1) was dramatically upregulated upon viral infection in the fish cell. We observed apoptosis in virus-infected cells and found that both PSR and IRF-1 increased simultaneously. Based on a bioinformatics promoter assay, IRF-1 binding sites were identified in the PSR promoter. Compared to normal viral infection, we found that PSR expression was delayed, viral replication was increased and virus-induced apoptosis was inhibited following IRF-1 suppression with morpholino oligonucleotides. A luciferase assay to analyze promoter activity revealed a decreasing trend after the deletion of the IRF-1 binding site on PSR promoter. The results of this study indicated that infectious pancreatic necrosis virus (IPNV) infection induced both the apoptotic and interferon (IFN) pathways, and IRF-1 was involved in regulating PSR expression to induce anti-viral effects. Therefore, this work suggests that PSR expression in salmonid cells during IPNV infection is activated when IRF-1 binds the PSR promoter. This is the first report to show the potential role of IRF-1 in triggering the induction of apoptotic cell clearance-related genes during viral infection and demonstrates the extensive crosstalk between the apoptotic and innate immune response pathways.
關聯: 15(10), pp.19281-19306
显示于类别:[水產養殖學系] 期刊論文


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