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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43440

Title: Piscidin-1, an Antimicrobial Peptide from Fish (Hybrid Striped Bass Morone saxatilis x M. chrysops), Induces Apoptotic and Necrotic Activity in HT1080 Cells
Authors: Heng-Ju Lin;Tsui-Chin Huang;Sasikala Muthusamy;Jheng-Fong Lee;Yeh-Fang Duann;Cheng-Hui Lin
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: antimicrobial peptides (AMPs);piscidin-1;cytotoxicity;apoptotic activity;necrotic activity
Date: 2012
Issue Date: 2017-08-08T07:56:55Z
Publisher: Zoological Science
Abstract: Abstract:Piscidin-1, a 22-residue cationic peptide isolated from mast cells of a hybrid striped bass, has potent antimicrobial activities against both gram-positive and -negative bacteria. To date, there is no report of its antitumor activity on any tumor cell lines. In this study, we examined the antitumor activity of a synthetic piscidin-1 peptide against several human cancer cell lines using an MTS assay and soft-agar colony-formation assay. We found that a low dose of piscidin induces both apoptosis and necrosis in HT1080 cells, as shown by annexin-V/propidium iodide and acridine orange/ethidium bromide staining, and triggers a necrotic cell death pathway in a short period with high-dose treatment. The destruction of cell membranes by piscidin-1 was demonstrated by transmission electron microscopy. Furthermore, piscidin-1 also inhibits the migration of HT1080 cells in a dose-dependent manner. This study provides the first evidence of the anticancer activity of the antimicrobial peptide, piscidin-1, with potential implications for the treatment of cancer.
Relation: 29, pp.327-332
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43440
Appears in Collections:[水產養殖學系] 期刊論文

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