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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43436

Title: A novel method for detection of fish iridovirus in groupers using nano-magnetic particles
Authors: Lu, Ming-Wei;Hsiang-Ping Kuo;Hsin-Yiu Chou;Ee-Ying Ling;Chia-Ling Chung, Yu-Fang Hung, Herng-Er Horng;Jen-Leih Wu;Guor-Mour Her;Zwe-Ling Kong
Contributors: 國立臺灣海洋大學:水產養殖學系
Date: 2016
Issue Date: 2017-08-08T06:47:03Z
Publisher: Fish Pathology
Abstract: Abstarct:Iridovirus is a serious aquatic virus infecting variable species of fish and frog. Development of a rapid, reliable detection method is an important issue for commercial and scientific purposes. With specific anti-body-functionalized magnetic nanoparticles, viruses can be bound immunologically and quantitatively detected by measuring the related magnetic signals. Immunomagnetic reduction (IMR) is a technique to measure the magnetic signals change in this work. Using this assay, the low-detection limit of iridovirus was found 101 TCID50/mL. There was no significant interference from the other grouper viruses including nervous necrosis virus (NNV). The iridovirus concentration in grouper detected with IMR was 91% related to real-time polymerase chain reaction (real-time PCR). These results demonstrated the feasibility of IMR on iridovirus screening in grouper.
Relation: 51, pp.S54-S59
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43436
Appears in Collections:[水產養殖學系] 期刊論文

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