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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43435

Title: Temperature- dependent Pathogenicity of Grouper Iridovirus of Taiwan (TGIV)
Authors: Liu, Hsin-I;Ying-Chu Lin;Pinwen Peter Chiou;Hsin-Yiu Chou
Contributors: 國立臺灣海洋大學:水產養殖學系
Date: 2016-06
Issue Date: 2017-08-08T03:01:54Z
Publisher: Journal of Marine Science and Technology-Taiwan
Abstract: Abstract:Grouper iridovirus of Taiwan (TGIV) is one of the most devastating infectious pathogens of grouper fry in Taiwan. As environmental temperature often plays an important role in the outbreak of diseases, we assayed the impact of different temperatures (18, 25 and 32 degrees C) on TGIV infection both in vitro and in vivo. Cytopathic effect (CPE) characterized as cell rounding and lysis were observed in TGIV-infected grouper SB (swim bladder) cells at 25 degrees C and 32 degrees C, but was absent at 18 degrees C. With temperature rising to 32 degrees C, the presence of pycnotic nuclei and chromatin margination became prominent in the infected cells, indicating an apoptotic death. The morphological feature of apoptotic cell was further supported by the observation under electron microscope. In addition, the apoptosis of TGIV-infected cells promoted by hyperthermia (32 degrees C) was confirmed by the assays of DNA laddering, DNA content and annexin V staining. To measure the temperature impact on the defense in TGIV-infected host, grouper fly was experimentally infected with the virus at 25 degrees C and 32 degrees C, respectively. Cumulative mortalities reached 100% in the fish held at 25 degrees C on day 10 post-infection, whereas only 37.5% at 32 degrees C at 2-week post-infection. In addition, the mortality in the infected fish of the 32-to-25 degrees C group skyrocketed to 97.5% by day 8. All together, the data suggest different impacts of temperatures on the pathogenicity of TGIV in grouper: while the lower temperature (18 degrees C) adversely affects the propagation of TGIV, the hyperthermic temperature (32 degrees C) promotes apoptosis to prevent the spreading of virus, resulting in higher resistance of the host against the virus.
Relation: 24(3), pp.637-644
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43435
Appears in Collections:[水產養殖學系] 期刊論文

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