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Title: Oocytes express an endogenous red fluorescent protein in a stony coral, Euphyllia ancora: a potential involvement in coral oogenesis
Authors: Shinya Shikina;Yi-Ling Chiu;Yi-Jou Chung;Chieh-Jhen Chen;Yan-Horn Lee;Ching-Fong Chang
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: Animal physiology;Reproductive biology
Date: 2016-05
Issue Date: 2017-08-08T01:48:11Z
Publisher: Scientific Reports
Abstract: Abstract:To date,the molecular and cellular mechanisms underlying coral sexual reproduction remain largely unknown. We then performed a differential screen to identify genes related to oogenesis in the stony coral Euphyllia ancora. We identified a clone encoding a novel red fluorescent protein cDNA of E. ancora (named EaRFP). Microscopic observation and quantitative RT-PCR revealed that EaRFP is almost exclusively expressed in the ovary of the adult coral. The combination of the ovarian-cell separation method and the RT-PCR analysis revealed that the oocytes, but not the ovarian somatic cells, are the cells expressing EaRFP. Immunohistochemical analysis revealed that the expression of EaRFP starts in the early stage of the oocyte and continues until the maturation period. Furthermore, recombinant EaRFP was shown to possess an H2O2 degradation activity. These results raise the possibility that EaRFP plays a role in protecting the oocytes from oxidative stress from the early to late stages of oogenesis. The present study provides not only the first evidence for the potential involvement of FPs in coral oogenesis but also an insight into a cellular strategy underlying coral sexual reproduction.
Relation: 6, Article number:25868
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43430
Appears in Collections:[水產養殖學系] 期刊論文

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