National Taiwan Ocean University Institutional Repository:Item 987654321/43389
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题名: Reaction of phosphorylase-a with α-D-glucose 1-phosphate and maltodextrin acceptors to give products with degree of polymerization 6-89
作者: Kazłowski, Bartosz;Ko, Yuan Tih
贡献者: 國立臺灣海洋大學:食品科學系
关键词: Degree of polymerization;Glucan saccharides;HPAEC-PAD;HPSEC-ELSD;Phosphorylase
日期: 2014-02-10
上传时间: 2017-07-25T06:13:56Z
出版者: Carbohydr Polym
摘要: Abstract:A series of linear glucan saccharides (GS) with defined quantity and degree of polymerization (DP) were synthesized from α-d-glucose 1-phosphate (α-d-Glc 1-P) by phosphorylase-a. The GS product fractions with average DP 11, 22, 38, 52, 60, 70, and 79 were measured by HPSEC-ELSD system. Then the same seven fractions were resolved into individual peaks with DP: 6-14, 10-32, 27-55, 37-67, 44-75, 49-83 and 53-89 by HPAEC-PAD system. Results showed that measurement of α-d-Glc 1-P amount consuming during GS synthesis by both systems enable calculation of reaction yield. The reaction yield for the 24h biosynthesis of the GS product was 25.3% (measured by HPSEC-ELSD) or 29.1% (measured by HPAEC-PAD). The HPSEC-ELSD and HPAEC-PAD systems were also successfully used for phosphorylase-a activity measurement in order to perform its kinetic characterization. This study established feasible systems for preparation of various sizes of the GS with defined DP and quantity as well as characterization of phosphorylase-a kinetics.
關聯: 106, pp.209-216
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43389
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