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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43370

Title: Isolation and characterization of collagens from the skin of giant grouper
Authors: Cheng-Hong Hsieh;Chyuan-Yuan Shiau;Yi-Cheng Su;Yi-Han Liu;Yu-Ru Huang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Giant grouper;Epinephelus lanceolatus;skin;acid solubilized collagen;pepsin solubilized collagen
Date: 2014-06-27
Issue Date: 2017-07-17T07:22:12Z
Publisher: Journal of Aquatic Food Product TTechnology
Abstract: Abstract:Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were extracted from the skin of giant groupers (Epinephelus lanceolatus) with yields of 39.51 and 19.12%, respectively. ASC and PSC consisted of two different α chains (α1 and α2) and were characterized to be type I collagen with no disulfide bond. The imino acid contents of the ASC and PSC from giant grouper skin were 189 and 181 per 1,000 residues, respectively. The maximum endothermic temperatures (Tmax) of ASC and PSC measured by differential scanning calorimetry (DSC) were 31.71 and 31.33°C, respectively. The denaturation temperatures of ASC and PSC measured by viscometry were 29.84 and 29.05°C, respectively. The maximum solubility in 0.5 M acetic acid was observed at pH 5 and pH 6 for ASC and PSC, respectively. A sharp decrease in solubility was observed for both ASC and PSC in the presence of NaCl above 3% (w/v).
Relation: 25(1), pp.93-104
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43370
Appears in Collections:[食品科學系] 期刊論文

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