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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43368

Title: Carotenoids composition in Scutellaria barbata D. Don as detected by high performance liquid chromatography-diode array detection-mass spectrometry-atmospheric pressure chemical ionization
Authors: Hsin-Lan Liu;Bing-Huei Chen;Tsai-Hua Kao;Chyuan-Yuan Shiau
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Scutellaria barbata D. Don;Carotenoids;HPLC-DAD-MS-APCI
Date: 2014-05
Issue Date: 2017-07-17T07:03:38Z
Publisher: Journal of Functional Foods
Abstract: Abstract:A high performance liquid chromatography-diode array detection-mass spectrometry method with atmospheric pressure chemical ionization mode (HPLC-DAD-MS-APCI) was developed to determine carotenoids in Scutellaria barbata D. Don (Banzhilian). Results showed that a total of 19 carotenoids including internal standard were separated within 37 min by employing a YMC C30 column and a gradient mobile phase composed of methanol–acetonitrile (86:14, v/v) and methylene chloride. The various carotenoids in S. barbata were identified and quantified using internal standard β-apo-8′-carotenal, in which all-trans-lutein and its cis isomers constituted the largest portion, followed by all-trans-β-carotene and its cis isomers. A high recovery of 93.0–100.9% and a high reproducibility were obtained with this method. This would allow determination of carotenoids in herbal samples, and provide a basis for possible production of functional foods with S. barbata D. Don as raw material.
Relation: 8, pp.100-110
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43368
Appears in Collections:[食品科學系] 期刊論文

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