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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43366

Title: Identification of species and measurement of tetrodotoxin in dried dressed fish fillets of Puffer fish
Authors: Tai-Yuan Chen;Yu-Wen Hsieh;Yung-Hsiang Tsai;Chyuan-Yuan Shiau;Deng-Fwu Hwang
Contributors: 國立臺灣海洋大學:食品科學系
Date: 2002-01-10
Issue Date: 2017-07-17T03:53:23Z
Publisher: Journal of Food Protection
Abstract: Abstract:The toxicity and species of dried dressed fish fillets were investigated. Three of seven dried dressed fish fillets were found to be toxic, with their levels of toxicity ranging from 9 to 18 mouse units per g. The toxin preparation was further purified and identified as tetrodotoxin and anhydrotetrodotoxin by high-performance liquid chromatography and gas chromatography–mass spectroscopy. The species of these fillets could not be distinguished from Lagocephalus lunaris by sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis or restriction site and sequence analysis of a polymerase chain reaction amplicon of the cytochrome b gene. These fish fillets were identified as L. lunaris on the basis of their protein patterns and gene characteristics. Furthermore, the toxic samples contained low levels of tetrodotoxin and its derivative.
Relation: 65(10), pp.1670-1673
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43366
Appears in Collections:[食品科學系] 期刊論文

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