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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43319

Title: Chemical and biological evaluation of nephrocizin in protecting nerve growth factor-differentiated PC12 cells by 6-hydroxydopamine-induced neurotoxicity
Authors: Yi-Pei Lin;Tai-Yuan Chen;Hsiang-Wen Tseng;Mei-Hsien Lee;Shui-Tein Chen
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Formosa date palm;Phoenix hanceana Naudin var. formosana;Arecaceae;6-Hydroxydopamine;H2O2;p-Quinone;Neuroprotection;Nephrocizin;Chemical and biological studies
Date: 2012-12
Issue Date: 2017-07-04T06:17:54Z
Publisher: Phytochemistry
Abstract: Abstract:The neurotoxin 6-hydroxydopamine (6-OHDA) has been widely used to generate an experimental model of Parkinson's disease. This model is crucial in the search for compounds that diminish 6-OHDA-induced nerve growth factor (NGF)-differentiated PC12 cell death. Nephrocizin (luteolin-7-O-β-D-glucopyranoside), a flavone glycoside, was isolated from widely distributed plants. The protective effects of pre-treatment with nephrocizin on the induced neurotoxicity in PC12 cells by 6-OHDA and its oxidative products, H₂O₂-, and p-quinone, were evaluated herein. Nephrocizin promoted cell viability, scavenged ROS-related products, increased cellular glutathione (GSH) levels, and reduced caspase-3 and -8 activities in 6-OHDA-, H₂O₂-, or p-quinone-treated PC12 cells. Furthermore, nephrocizin-conjugated metabolites in PC12 cells were identified with the boronate-affinity method and LC-MS technology, and preferential regioselectivity at the C2' and C5' positions by the nephrocizin-GSH (or NAC) adduct method was observed. These lines of evidence established that nephrocizin could form a dimer to diminish the intracellular ROS. These results demonstrate the first neuroprotective mechanism of nephrocizin against 6-OHDA-, H₂O₂- or p-quinone-induced cytotoxicity in PC12 cells via chemical and biological studies. These dietary antioxidants are potential candidates for use in intervention in neurodegenerative diseases.
Relation: 84, pp.102-115
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43319
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