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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43313

Title: Proteomic identification of stonefish Synanceja verrucosa venom
Authors: Tai-Yuan Chen;Yu-Huai Chang;Hsi-Pin Lin;Shui-Tein Chen;Deng-Fwu Hwang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: 2-DE;MALDI-Q-TOF;neoverrucotoxin;spine venom;Synanceja verrucosa
Date: 2015
Issue Date: 2017-07-04T02:06:41Z
Publisher: Journal of Food and Nutrition Research
Abstract: Abstract:Three venom toxins, neoverrucotoxin (neoVTX) α-subunit and β-subunit as well as verrucotoxin (VTX) β-subunit, were identified in the stonefish Synanceja verrucosa by SDS-PAGE, Native-PAGE and two-dimensional electrophoresis (2-DE) coupled with Matrix Assisted Laser Desorption Ionization-Quadrupole-Time-of-Flight (MALDI-Q-TOF). The venom estimated by Native-PAGE were 471, 358, 260 and 166 kDa. The predominate protein bands of crude venom were 84 and 75 kDa by SDS-PAGE. The crude venom protein fell in the region with pI values of 7-9 and molecular weights of 75-90 kDa by 2-DE. Peptide mass fingerprints (PMF) and MS/MS ions originated from MALDI-Q-TOF were used to identify the protein. Our results showed that the complete components of neoverrucotoxin (neoVTX) α-subunit and β-subunit as well as verrucotoxin (VTX) β-subunit were identified from SDS-PAGE and 2-DE patterns. Native-PAGE did not yield protein identifications but revealed the presence of protein complexes.
Relation: 3(8), pp.526-539
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43313
Appears in Collections:[食品科學系] 期刊論文

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