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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43310

Title: Development of standardized methodology for identifying toxins in clinical samples and fish species associated with tetrodotoxin-borne poisoning incidents
Authors: Tai-Yuan Chen;Cheng-Hong Hsieh;Deng-Fwu Hwang
Contributors: 國立臺灣海洋大學:河海工程學系
Keywords: identification;liquid chromatography–tandem mass spectrometry;polymerase chain reaction method coupled with restriction fragment length polymorphism;tetrodotoxin;tetrodotoxin poisoning incident
Date: 2016-01
Issue Date: 2017-06-23T06:12:07Z
Publisher: Journal of Food and Drug Analysis
Abstract: Abstract:Tetrodotoxin (TTX) is a naturally occurring toxin in food, especially in puffer fish. TTX poisoning is observed frequently in South East Asian regions. In TTX-derived food poisoning outbreaks, the amount of TTX recovered from suspicious fish samples or leftovers, and residual levels from biological fluids of victims are typically trace. However, liquid chromatography–mass spectrometry and liquid chromatography–tandem mass spectrometry methods have been demonstrated to qualitatively and quantitatively determine TTX in clinical samples from victims. Identification and validation of the TTX-originating seafood species responsible for a food poisoning incident is needed. A polymerase chain reaction-based method on mitochondrial DNA analysis is useful for identification of fish species. This review aims to collect pertinent information available on TTX-borne food poisoning incidents with a special emphasis on the analytical methods employed for TTX detection in clinical laboratories as well as for the identification of TTX-bearing species.
Relation: 24(1), pp.9-14
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43310
Appears in Collections:[河海工程學系] 期刊論文

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