English  |  正體中文  |  简体中文  |  Items with full text/Total items : 26988/38789
Visitors : 2312591      Online Users : 30
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43272

Title: Purification and identification of hypocholesterolemic peptides from freshwater clam hydrolysate with in vitro gastrointestinal digestion
Authors: Yu-Hsin Lin;Jenn-Shou Tsa;Guan-Wen Chen
Contributors: 國立臺灣海洋大學:食品科學系
Date: 2017-05-18
Issue Date: 2017-06-15T01:26:15Z
Abstract: Abstract:The muscles of freshwater clams were extracted separately using hot water. Subsequently, the edible muscle part was freeze-dried, hydrolyzed at 50°C using Protamex to obtain the freshwater clam hydrolysate (PX), and then digested with pepsin. The bile-acid-binding capacity and inhibition of cholesterol micelle formation were subsequently investigated using pepsin-digested PX (PXP) through ultrafiltration (UF) fractionation or size exclusion chromatography. After the UF treatment, soluble protein (171.0 mg/g) and peptide (109.4 mg/g) contents in fraction III were found to be higher than those of all other membrane fractions. Assuming that bile acid binds to PXP at 100%, the relative bile-acid-binding capacities of fractions I, II, and III were 161.2%, 64.3%, and 55.1%, respectively. Fraction f showed the highest inhibitory efficiency ratio (IER), and its inhibition-peptide content percentage was 831.5% mg/mL. The amino acid sequences of two hypocholesterolemic peptides were Val–Lys–Pro and Val–Lys–Lys, with IERs of 64.8% and 10.2% mg/mL, respectively.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/43272
Appears in Collections:[食品科學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML84View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback