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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41514

Title: Phytoplankton Pigment Analysis by HPLC and its Application in Algal Community Investigations
Authors: Hsiu-Ping;Gwo-Ching Gong;Tung-Ming Hsiung
Contributors: 國立臺灣海洋大學:海洋環境化學與生態研究所
Date: 2002-10
Issue Date: 2017-02-24T07:05:25Z
Publisher: Botanical Bulletin of Academia Sinica
Abstract: Abstract:A high performance liquid chromatography (HPLC) system for pigment analysis of marine phytoplankton was established. The characteristic quantification limits of the major photosynthetic chlorophylls a and b were 0.29 and 0.27 ng, respectively. The reliability of this method was verified by resolving the marker pigments in culture extracts from diatoms, blue-green algae and green algae. Fucoxanthin, zeaxanthin and chlorophyll b were selected for calculating the chlorophyll a/marker pigment ratios for the three types of algae, which were 1.39, 0.51 and 1.01, respectively. The composition of the phytoplankton community in summer samples, collected from the continental shelf of the East China Sea, was estimated by simple regression of these ratios. When results of composition abundance of diatoms and blue-green algae obtained by this method were compared with those obtained by the microscopic technique, the relative percentage difference was less than 6% for diatoms. On the other hand, a lower abundance of blue-green algae was estimated by the HPLC method.
Relation: 43(4), pp.283-290
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41514
Appears in Collections:[海洋環境與生態研究所] 期刊論文

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