English  |  正體中文  |  简体中文  |  Items with full text/Total items : 28328/40321
Visitors : 4093522      Online Users : 113
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41514

Title: Phytoplankton Pigment Analysis by HPLC and its Application in Algal Community Investigations
Authors: Hsiu-Ping;Gwo-Ching Gong;Tung-Ming Hsiung
Contributors: 國立臺灣海洋大學:海洋環境化學與生態研究所
Date: 2002-10
Issue Date: 2017-02-24T07:05:25Z
Publisher: Botanical Bulletin of Academia Sinica
Abstract: Abstract:A high performance liquid chromatography (HPLC) system for pigment analysis of marine phytoplankton was established. The characteristic quantification limits of the major photosynthetic chlorophylls a and b were 0.29 and 0.27 ng, respectively. The reliability of this method was verified by resolving the marker pigments in culture extracts from diatoms, blue-green algae and green algae. Fucoxanthin, zeaxanthin and chlorophyll b were selected for calculating the chlorophyll a/marker pigment ratios for the three types of algae, which were 1.39, 0.51 and 1.01, respectively. The composition of the phytoplankton community in summer samples, collected from the continental shelf of the East China Sea, was estimated by simple regression of these ratios. When results of composition abundance of diatoms and blue-green algae obtained by this method were compared with those obtained by the microscopic technique, the relative percentage difference was less than 6% for diatoms. On the other hand, a lower abundance of blue-green algae was estimated by the HPLC method.
Relation: 43(4), pp.283-290
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41514
Appears in Collections:[海洋環境與生態研究所] 期刊論文

Files in This Item:

File Description SizeFormat

All items in NTOUR are protected by copyright, with all rights reserved.


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback