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Title: Expression of death-related genes and reactive oxygen species production in Skeletonema tropicum upon exposure to the polyunsaturated aldehyde octadienal
Authors: Raffaella Casotti
Alessandra A. Gallina
Chih-Ching Chung
Contributors: 國立臺灣海洋大學:海洋環境化學與生態研究所
Keywords: ROS
chemical ecology
bloom evolution
Programmed cell death
Date: 2015-11
Issue Date: 2017-02-10
Publisher: Advances in Oceanography and Limnology
Abstract: Abstract: The effects of 4E/Z-octadienal (OCTA) on ScDSP-1 and ScDSP-2 gene expression and reactive oxygen species (ROS) production were investigated in the marine diatom Skeletonema tropicum (formerly costatum) using qRTPCR and flow cytometry. ScDSP-1 and ScDSP-2 genes have been previously shown to be involved in cell death in ageing cells and in response to photosynthetic stress. OCTA induced a differential, concentration-dependent DSP gene expression associated to ROS production, 821.6 and 97.7 folds higher for ScDSP-1 and ScDSP-2, respectively. Among the concentrations tested, only 8 μM OCTA, which caused a reduction of 50% in cell concentrations at 24 h, was able to elicit an expression pattern consistent with a signalling role. Interestingly, only intermediate levels of reactive oxygen species (ROS) (i.e., 1.5±0.1 increase) were observed to be elicited by such concentration. These results suggest that ROS are key components of the molecular cascade triggered by polyunsaturated aldehydes (PUA) and leading to cell death. This could have implications for bloom final stages at sea, where PUA may act as effectors of diatom population dynamics through ROS acting as modulators.
Relation: 6(1/2)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41181
Appears in Collections:[海洋環境與生態研究所] 期刊論文

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