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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41178

Title: A novel transforming growth factor-β superfamily member expressed in gonadal somatic cells enhances primordial germ cell and spermatogonial proliferation in rainbow trout (Oncorhynchus mykiss)
Authors: Goro Yoshizaki
Etsuko Sawatari
Shinya Shikina
Toshio Takeuchi
Contributors: 國立臺灣海洋大學:海洋環境化學與生態研究所
Keywords: Trout
Sertoli cell
Date: 2007-02
Issue Date: 2017-02-09T08:41:56Z
Publisher: Developmental Biology
Abstract: Abstract: Our understanding of the molecular mechanisms of primordial germ cell (PGC) proliferation in fish is rudimentary, but it is thought to be controlled by the surrounding somatic cells. We assumed that growth factors that are specifically involved in PGC proliferation are expressed predominantly in the surrounding genital ridge somatic cells. In order to isolate these growth factors, we compiled a complementary DNA (cDNA) subtractive library using cDNA from the genital ridges of 40-dpf rainbow trout embryos as the tester and cDNA from embryos without genital ridges as the driver. This approach identified a novel cytokine, designated gonadal soma-derived growth factor (GSDF), which is a member of the transforming growth factor (TGF)-β superfamily. GSDF was expressed in the genital ridge somatic cells surrounding the PGCs during embryogenesis, and in both the granulosa and Sertoli cells at later stages. Inhibition of GSDF translation by antisense oligonucleotides suppressed PGC proliferation. Moreover, isolated testicular cells that were cultured with recombinant GSDF demonstrated dose-dependent proliferation of type-A spermatogonia; this effect was completely blocked by antiserum against GSDF. These results denote that GSDF, a novel member of the TGF-β superfamily, plays an important role for proliferation of PGC and spermatogonia.
Relation: 301(1)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41178
Appears in Collections:[海洋環境與生態研究所] 期刊論文

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