English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27228/39071
Visitors : 2405370      Online Users : 60
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41167

Title: Spermatogonial transplantation in fish: A novel method for the preservation of genetic resources
Authors: Yutaka Takeuchi
Goro Yoshizaki
Kiyoko Fujinuma
Yoshiko Iwasaki
Tomoyuki Okutsu
Shinya Shikina
Ryosuke Yazawa
Contributors: 國立臺灣海洋大學:海洋環境化學與生態研究所
Keywords: Trout
Cryopreservation
Germ cell
Salmon
Spermatogonia
Transplantation
Triploid
Date: 2011-03
Issue Date: 2017-02-09T07:56:18Z
Publisher: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
Abstract: Abstract: Recent progress in genome-based breeding has created various fish strains carrying desirable genetic traits; however, methods for the long-term preservation of their genetic resources have not yet been developed, mainly due to the lack of cryopreservation techniques for fish eggs and embryos. Recently, we established an alternative cryopreservation technique for fish spermatogonia using a slow-freezing method. Furthermore, we developed a transplantation system to produce functional eggs and sperm derived from spermatogonia. Spermatogonia isolated from the testes of vasa-green fluorescent protein (Gfp) transgenic rainbow trout (Oncorhynchus mykiss) were transplanted into the peritoneal cavity of triploid masu salmon (Oncorhynchus masou) hatchlings of both genders. The transplanted trout spermatogonia migrated towards the gonadal anlagen of the recipient salmon, into which they were subsequently incorporated. We confirmed that the donor-derived spermatogonia resumed gametogenesis, and produced sperm and eggs in male and female recipient salmon, respectively. Fertilization of the resultant eggs and sperm produced only rainbow trout in the first filial (F1) generation, suggesting that the sterile triploid recipient salmon produced functional eggs and sperm derived from the trout donors. A combination of spermatogonial transplantation and cryopreservation could be a powerful tool for preserving valuable fish strains with desirable genetic traits and endangered species.
Relation: 6(1)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41167
Appears in Collections:[海洋環境與生態研究所] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML65View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback