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题名: Yolk formation in a stony coral Euphyllia ancora (Cnidaria, Anthozoa): insight into the evolution of vitellogenesis in non-bilaterian animals
作者: Ching-Fong Chang
Shinya Shikina
Chieh-Jhen Chen
Yi-Jou Chung
Zi-Fan Shao
Jhe-Yu Liou
Hua-Pin Tseng
Yan-Horn Lee
贡献者: 國立臺灣海洋大學:海洋環境化學與生態研究所
日期: 2013-06
上传时间: 2017-02-09T07:16:14Z
出版者: Endocrinology
摘要: Abstract: Vitellogenin (Vg) is a major yolk protein precursor in numerous oviparous animals. Numerous
studies in bilateral oviparous animals have shown that Vg sequences are conserved across taxa and
that Vgs are synthesized by somatic-cell lineages, transported to and accumulated in oocytes,
and eventually used for supporting embryogenesis. In nonbilateral animals (Polifera, Cnidaria, and
Ctenophora), which are regarded as evolutionarily primitive, although Vg cDNA has been identified
in 2 coral species from Cnidaria, relatively little is known about the characteristics of yolk
formation in their bodies. To address this issue, we identified and characterized 2 cDNA encoding
yolk proteins,Vg and egg protein (Ep), in the stony coral Euphyllia ancora. RT-PCR analysis revealed
that expression levels of both Vg and Ep increased in the female colonies as coral approached the
spawning season. In addition, high levels of both Vg and Ep transcripts were detected in the
putative ovarian tissue, as determined by tissue distribution analysis. Further analyses using mRNA
in situ hybridization and immunohistochemistry determined that, within the putative ovarian
tissue, these yolk proteins are synthesized in the mesenterial somatic cells but not in oocytes
themselves. Furthermore, Vg proteins that accumulated in eggs were most likely consumed during
the coral embryonic development, as assessed by immunoblotting. The characteristics of Vg that
we identified in corals were somewhat similar to those of Vg in bilaterian oviparous animals, raising
the hypothesis that such characteristics were likely present in the oogenesis of some common
ancestor prior to divergence of the cnidarian and bilaterian lineages.
關聯: 154(9)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41161
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