English  |  正體中文  |  简体中文  |  Items with full text/Total items : 28611/40649
Visitors : 638441      Online Users : 78
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41161

Title: Yolk formation in a stony coral Euphyllia ancora (Cnidaria, Anthozoa): insight into the evolution of vitellogenesis in non-bilaterian animals
Authors: Ching-Fong Chang
Shinya Shikina
Chieh-Jhen Chen
Yi-Jou Chung
Zi-Fan Shao
Jhe-Yu Liou
Hua-Pin Tseng
Yan-Horn Lee
Contributors: 國立臺灣海洋大學:海洋環境化學與生態研究所
Date: 2013-06
Issue Date: 2017-02-09T07:16:14Z
Publisher: Endocrinology
Abstract: Abstract: Vitellogenin (Vg) is a major yolk protein precursor in numerous oviparous animals. Numerous
studies in bilateral oviparous animals have shown that Vg sequences are conserved across taxa and
that Vgs are synthesized by somatic-cell lineages, transported to and accumulated in oocytes,
and eventually used for supporting embryogenesis. In nonbilateral animals (Polifera, Cnidaria, and
Ctenophora), which are regarded as evolutionarily primitive, although Vg cDNA has been identified
in 2 coral species from Cnidaria, relatively little is known about the characteristics of yolk
formation in their bodies. To address this issue, we identified and characterized 2 cDNA encoding
yolk proteins,Vg and egg protein (Ep), in the stony coral Euphyllia ancora. RT-PCR analysis revealed
that expression levels of both Vg and Ep increased in the female colonies as coral approached the
spawning season. In addition, high levels of both Vg and Ep transcripts were detected in the
putative ovarian tissue, as determined by tissue distribution analysis. Further analyses using mRNA
in situ hybridization and immunohistochemistry determined that, within the putative ovarian
tissue, these yolk proteins are synthesized in the mesenterial somatic cells but not in oocytes
themselves. Furthermore, Vg proteins that accumulated in eggs were most likely consumed during
the coral embryonic development, as assessed by immunoblotting. The characteristics of Vg that
we identified in corals were somewhat similar to those of Vg in bilaterian oviparous animals, raising
the hypothesis that such characteristics were likely present in the oogenesis of some common
ancestor prior to divergence of the cnidarian and bilaterian lineages.
Relation: 154(9)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41161
Appears in Collections:[海洋環境與生態研究所] 期刊論文

Files in This Item:

File Description SizeFormat

All items in NTOUR are protected by copyright, with all rights reserved.


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback