National Taiwan Ocean University Institutional Repository:Item 987654321/41155
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题名: Molecular cloning and characterization of a steroidogenic enzyme, 17b-hydroxysteroid dehydrogenase type 14, from the stony coral Euphyllia ancora (Cnidaria, Anthozoa)
作者: Ching-Fong Chang
Yi-Jou Chung
Shinya Shikina
Yi-Ling Chiu
Yi-Jie Huang
Yan-Horn Lee
贡献者: 國立臺灣海洋大學:海洋環境化學與生態研究所
关键词: Steroidogenic enzyme
Coral
Cnidarian
Euphyllia ancora
17β-Hydroxysteroid dehydrogenase 14
日期: 2016-03
上传时间: 2017-02-09T06:13:53Z
出版者: General and Comparative Endocrinology
摘要: Abstract: Sex steroids play a fundamental role not only in reproduction but also in various other biological processes in vertebrates. Although the presence of sex steroids has been confirmed in cnidarians (e.g., coral, sea anemone, jellyfish, and hydra), which are basal metazoans, only a few studies to date have characterized steroidogenesis-related genes in cnidarians. Based on a transcriptomic analysis of the stony coral Euphyllia ancora, we identified the steroidogenic enzyme 17β-hydroxysteroid dehydrogenase type 14 (17beta-hsd 14), an oxidative enzyme that catalyzes the NAD+-dependent inactivation of estrogen/androgen (estradiol to estrone and testosterone to androstenedione) in mammals. Phylogenetic analysis showed that E. ancora 17beta-Hsd 14 (Ea17beta-Hsd 14) clusters with other animal 17beta-HSD 14s but not with other members of the 17beta-HSD family. Subsequent quantitative RT-PCR analysis revealed a lack of correlation of Ea17beta-hsd 14 transcript levels with the coral’s reproductive cycle. In addition, Ea17beta-hsd 14 transcript and protein were detected in all tissues examined, such as the tentacles, mesenterial filaments, and gonads, at similar levels in both sexes, as determined by quantitative RT-PCR analysis and Western blotting with an anti-Ea17beta-Hsd 14 antibody. Immunohistochemical analysis revealed that Ea17beta-Hsd 14 is mainly distributed in the endodermal regions of the polyps, but the protein was also observed in all tissues examined. These results suggest that Ea17beta-Hsd 14 is involved in important functions that commonly occur in endodermal cells or has multiple functions in different tissues. Our data provide information for comparison with advanced animals as well as insight into the evolution of steroidogenesis-related genes in metazoans.
關聯: 228
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41155
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