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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41151

Title: Cross-shelf variation of nitrate transporter messenger RNA level as an indication of diatom nitrogen stress
Authors: Jeng Chang;Lee-Kuo Kang
Contributors: 國立臺灣海洋大學:海洋環境化學與生態研究所
Date: 2012-07
Issue Date: 2017-02-09T03:26:47Z
Publisher: 2012 ASLO Aquatic Sciences Meeting
Abstract: Diatoms are the major primary producers supporting the marine grazing food chain, and their growths in the sea were frequently reported as limited by nitrogenous nutrients. Recently, a group of nitrate transporter genes (Nrt2) was proposed as a potential marker for evaluating nitrogen deficiencies in diatoms. The mRNA levels of Nrt2 are repressed in the presence of ammonium, and highly expressed in nitrogen-deprived condition. In the summer of 2010, an oceanographic cruise was conducted in the southern East China Sea to investigate the cross-shelf variation of nitrogen stress experienced by diatoms. Based on results obtained by quantitative reverse-transcription polymerase chain reaction, moderate to low levels of Nrt2 mRNA occurred in the coastal zone. In addition, high levels of Nrt2 mRNA were observed in the mid-shelf zone but the mRNA level decreased again toward an upwelling system located at the shelf break. Comparisons between values from untreated samples and those with nitrogen manipulations indicated that, in natural environments, nitrogen stresses in diatoms were effectively released by either terrestrial runoff or upwelling waters.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41151
Appears in Collections:[海洋環境與生態研究所] 演講及研討會

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