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Title: Diversity of diatom silicon transporter sequences and their expressions during a Changjiang River flooding event in the East China Sea
Authors: Jeng Chang
Lee-Kuo Kang
Chao-Chang Feng
Contributors: 國立臺灣海洋大學:海洋環境化學與生態研究所
Date: 2014-05
Issue Date: 2017-02-09T03:09:59Z
Abstract: Diatoms are successful phytoplankton responsible for 75% of the primary production occurring in high-nutrient and coastal regions of the ocean, and their growth is sometimes limited by the silicon availability. We investigated the genetic diversity of the silicon transporter genes (Sit) and measured Sit gene expression in one genus, Chaetoceros, as a molecular indicator of silicon deficiency in the East China Sea (ECS). Sit gene diversity was evaluated by sequencing 34 new Sit fragments from cultured diatoms spanning 12 genera. With the aid of this expanded sequence database, the taxonomical affiliation of ECS Sit sequences were determined. According to the alignment of Sit sequences, Chaetoceros-specific primers were designed for detecting Sit mRNA levels by quantitative RT-PCR. In a cruise conducted in July 2010, the Chaetoceros Sit transcript levels were all lower than the threshold of silicate deficiency established from a cultured C. affinis, indicating Chaetoceros spp. were not limited by silicate in our study area. This could be due to a devastating flood event of the Changjiang River in 2010 that exported large amounts of silicic acid to the ECS.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41147
Appears in Collections:[海洋環境與生態研究所] 演講及研討會

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