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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41128

Title: Community structure of planktonic methane-oxidizing bacteria in a subtropical reservoir characterized by dominance of phylotype closely related to nitrite reducer
Authors: Manabu Fukui
Hisaya Kojima
Riho Tokizawa
Kouhei Kogure
Yuki Kobayashi
Masayuki Itoh
Fuh-Kwo Shiah
Noboru Okuda
Contributors: 國立臺灣海洋大學:海洋環境化學與生態研究所
Date: 2014
Issue Date: 2017-02-09
Publisher: Scientific Reports
Abstract: Abstract: Methane-oxidizing bacteria (MOB) gain energy from the oxidation of methane and may play important roles in freshwater ecosystems. In this study, the community structure of planktonic MOB was investigated in a subtropical reservoir. Bacterial community structure was investigated through the analysis of the 16S rRNA gene. Three groups of phylogenetically distinct MOB were detected in the clone libraries of polymerase chain reaction products obtained with universal primers. The groups belonged to the class Gammaproteobacteria, the class Alphaproteobacteria, and the candidate phylum NC10. The last group, which consists of close relatives of the nitrite reducer ‘Candidatus Methylomirabilis oxyfera’, was frequently detected in the clone libraries of deep-water environments. The presence of 3 groups of MOB in deep water was also shown by a cloning analysis of the pmoA gene encoding particulate methane monooxygenase. The dominance of ‘M. oxyfera’-like organisms in deep water was confirmed by catalyzed reporter deposition–fluorescence in situ hybridization, in which cells stained with a specific probe accounted for 16% of total microbial cells. This is the first study to demonstrate that close relatives of the nitrite reducer can be major component of planktonic MOB community which may affect carbon flow in aquatic ecosystems.
Relation: 4
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/41128
Appears in Collections:[海洋環境與生態研究所] 期刊論文

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