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题名: Development and evaluation of a sensitive enzyme-linked oligosorbent assay for detection of polymerase chain reaction amplified hepatitis C virus of genotypes 1 to 6
作者: Huang RY;Chang HT;Lan CY;Pai TW;Wu CN;Ling CM;Chang MD.
贡献者: 國立臺灣海洋大學:資訊工程學系
日期: 2008
上传时间: 2017-01-16T07:07:09Z
出版者: J Virol Methods.
摘要: Abstract: A high-throughput polymerase chain reaction (PCR)-based enzyme-linked oligonucleotide-sorbent assay (ELOSA) was developed for use in the diagnostic testing of serum from patients who may be infected with different hepatitis C virus (HCV) genotypes. Twelve genotype-specific 5'-aminated DNA-coated probes were designed based on the variable 5'-untranslated region sequences of the HCV genotypes 1-6. Using 100 clinical serum samples, the performance of the PCR-ELOSA method was compared with Roche's COBAS Amplicor HCV Monitor V2.0 assay and the VERSANT HCV genotype assay (LiPA), and the overall agreement was 99% at the level of HCV genotypes with a detection range of 2.0 x 10(2) to 1.0 x 10(7)IU/ml for PCR-ELOSA. The PCR-ELOSA was more comprehensive as demonstrated by the fact that approximately 20% of the samples with different subtypes could be discriminated by this method but not by LiPA. In addition, the PCR-ELOSA system showed high accuracy (CV<or=6.36%) and even higher reproducibility (CV<or=5.55%). Thus, this novel PCR-ELOSA system provides a sensitive and versatile alternative to current HCV detection assays.
關聯: 151(2)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/40238
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