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|Title: ||Nitrogen excretion and changes of hemocyanin, protein and free amino acid levels in the hemolymph of Penaeus monodon exposed to different concentrations of ambient ammonia-N at different salinity levels|
|Authors: ||Jiann-Chu Chen;C-T Chen;S-Y Cheng|
|Issue Date: ||2016-12-26T07:23:33Z
|Publisher: ||Marine Ecology Progress Series|
|Abstract: ||Abstract:Penaeus monodon (22.064 * 2.263 g) were exposed individually at 10, 20 and 30% salin-ity to 0 (control), 1, 5, 10 and 20 mg 1-' ammonia-N for 24 h. Changes in hemocyanin, protein and free amino acid levels, and whole shrimp ammonia-N excretion, urea-N excretion and dissolved organic N (DON) excretion were determined. Ammonia-N excretion decreased with increased salinity and with increased ambient ammonia-N. Ammonia-N accounted for 56.87. 70.43 and 78.13 % of total nitrogen excreted by P. monodon in 30, 20 and 10% respectively. DON increased to 67.26, 78.79 and 86.1% of total nitrogen excreted by the shrimp exposed to 20 mg 1-' ammonia-N in 30, 20 and 10°A, respectively. Net ammonia-N uptake occurred as shrimp were exposed to ambient ammonia-N greater than 5 mg 1-I at all salinity levels tested. Hemocyanin and protein levels decreased, whereas hemolyrnph ammonia, urea, total free amino acid levels and taurine level increased, with increased ambient ammonia-N. Following exposure to ambient ammonia-N, P. rnonodon changed its excretory pattern, accumulation of hemolymph ammonia, and catabolism of hemocyanin and protein to balance osmoregulation.|
|Relation: ||110(1), pp.85-94|
|Appears in Collections:||[環境生物與漁業科學學系] 期刊論文|
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