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題名: Recovery of Penaeus monodon from functional anaemia after exposure to sublethal concentration of nitrite at different pH levels
作者: Jiann-Chu Chen;Sha-Yen Cheng
貢獻者: 國立臺灣海洋大學:環境生物與漁業科學學系
關鍵詞: Penaeus monodon;Nitrite;pH;Hemolymph;Depuration;Recovery;Oxyhemocyanin;Hemolymph protein;Osmolality
日期: 2000-08
上傳時間: 2016-12-26T06:35:24Z
出版者: Aquatic Toxicology
摘要: Abstract:Tiger shrimp Penaeus monodon (14.79±0.19 g) which had been exposed individually to 0.72 mM nitrite at pH 6.8, 8.2 and 9.8 after 3, 6, 12, 24 and 48 h were examined for the water nitrite concentration, hemolymph nitrite, oxyhemocyanin, protein and osmolality levels. Same parameters were examined for shrimp depurated in nitrite-free water after 3, 6, 12 and 24 h following 48 h exposure to nitrite. In the nitrite-exposed test, nitrite influx, hemolymph nitrite and osmotic differential (medium osmolality — hemolymph osmolality) increased with exposure time, and were higher at pH 6.8, whereas water nitrite concentration, oxyhemocyanin, protein, ratio of oxyhemocyanin to protein and hemolymph osmolality decreased with exposure time and were lower at pH 6.8. In the depuration test, water nitrite concentration increased with depuration time, and was higher at pH 6.8, whereas, hemolymph oxyhemocyanin, ratio of oxyhemocyanin to protein and hemolymph osmolality increased with depuration time and were higher at pH 9.8. Hemolymph nitrite decreased with depuration time, and was 2.52, 2.19 and 0.24 μmol ml−1 after 6 h at pH 6.8, 8.2 and 9.8, respectively. Nitrite entry is considered to occur mainly via the form of nitrous acid (HNO2) in water uptake. The fact that the hemolymph nitrite decreased to non-detectable level with 72–88% recovery of oxyhemocyanin after 24 h depuration suggests an occurrence of reductase for nitrite-exposed shrimps.
關聯: 50(1-2), pp.73-83
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39644
顯示於類別:[環境生物與漁業科學學系] 期刊論文

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