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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39560

Title: Rapid detection of natriuretic peptides by a microfluidic LabChip analyzer with DNA aptamers: Application of natriuretic peptide detection
Authors: Shui-Tein Chen
Ming-Cheng Lin
Jiraporn Nawarak
Tai-Yuan Chen
Hsien-Yu Tsai
Jung-Feng Hsieh
Supachok Sinchaikul
Contributors: 國立臺灣海洋大學:食品科學系
Date: 2009-09
Issue Date: 2016-12-21T03:49:39Z
Publisher: Biomicrofluidics
Abstract: Abstract: Rapid detection of brain natriuretic peptide (BNP) concentration can be used for the diagnosis of acute heart failure and for the evaluation of the effectiveness of a clinical therapy. We used the systematic evolution of ligands by exponential enrichment method to develop DNA aptamers for BNP whose sequences were determined by cloning method and consensus sequence analysis. A total of eight conserved sequences was identified. By combining the fluorescent-labeled aptamers with fast protein lab-on-chip analysis, we could achieve quantification of BNP concentrations with high speed, sensitivity, and specificity.
Relation: 3(3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39560
Appears in Collections:[食品科學系] 期刊論文

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