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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39557

Title: Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line
Authors: Jen-Hwey Chiu;Chun-Ju Chang;Jing-Chong Wu;Hui-Ju Liu;Che-Sheng Wen;Chung-Hua Hsu;Jiun-Liang Chen;Ling-Ming Tseng;Wei-Shone Chen;Yi-Ming Shyr
Contributors: 國立臺灣海洋大學:食品科學系
Date: 2014
Issue Date: 2016-12-21T03:08:09Z
Publisher: Evidence-Based Complementary and Alternative Medicine
Abstract: Abstract: Aim. Our aim the was to screen the commonly used Chinese herbs in order to detect changes in ERBB2 and ESR1 gene expression using MCF-7 cells. Methods. Using the MCF-7 human breast cancer cell line, cell cytotoxicity and proliferation were evaluated by MTT and trypan blue exclusion assays, respectively. A luciferase reporter assay was established by transient transfecting MCF-7 cells with plasmids containing either the ERBB2 or the ESR1 promoter region linked to the luciferase gene. Chinese herbal extracts were used to treat the cells at 24 h after transfection, followed by measurement of their luciferase activity. The screening results were verified by Western blotting to measure HER2 and ERα protein expression. Results. At concentrations that induced little cytotoxicity, thirteen single herbal extracts and five compound recipes were found to increase either ERBB2 or ESR1 luciferase activity. By Western blotting, Si-Wu-Tang, Kuan-Shin-Yin, and Suan-Tsao-Ren-Tang were found to increase either HER2 or ERα protein expression. In addition, Ligusticum chuanxiong was shown to have a great effect on ERBB2 gene expression and synergistically with estrogen to stimulate MCF-7 cell growth. Conclusion. Our results provide important information that should affect clinical treatment strategies among breast cancer patients who are receiving hormonal or targeted therapies.
Relation: 2014
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39557
Appears in Collections:[食品科學系] 期刊論文

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