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Title: Enzymes-hydrolyzed Fucooligosaccharides from Sargassum siliquosum and Their Intestinal Transportation Across Human Caco-2 Cells
Authors: Chun-Ju Chang
Chorng-Liang Pan
Shao-Chiang Chang
Chaio Lee
Po-Hsuan Chen
Bartosz Kazlowski
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Sargassum siliquosum
fucooligosaccharide (FOS)
Caco-2 cell
intestinal absorption and transport
Date: 2015
Issue Date: 2016-12-21T02:47:27Z
Publisher: Journal of The Fisheries Society of Taiwan
Abstract: Abstract: Fucooligosaccharide is one of the major active components in brown algae. Fucooligosaccharide (FOS) possesses antioxidative, anti-coagulation and antiviral effects, but its intestinal bioavailability needs siliquosum and to determine their absorption and transportation in human enterocytes. Exploring the absorption and transportation of the Fuc-S1 and Fuc-S2 mixture by using the in vitro Caco-2 monolayers confirmed that the concentration of Fuc-S1 in the apical fluid decreased from 3.71 ± 0.73 mg/ml to 3.04 ± 0.34 mg/ml, whereas the concentration in the basolateral fluid increased from 2.33 ± 0.31 mg/ml to 2.80 ± 0.53 mg/ml. Therefore, the amount of Fuc-S1 absorbed by the Caco-2 monolayers was approximately 4.07 ± 0.60 mg/ml. Furthermore, the concentration of Fuc-S2 in the upper layer solution ecreased from 2.93 ± 0.24 mg/ml to 2.14 ± 0.86 mg/ml, whereas the concentration in the lower layer solution increased from 2.15 ± 0.18 mg/ml to 3.10 ± 0.27 mg/ml. Accordingly, the amount of Fuc-S2 absorbed by the Caco-2 monolayers was approximately 4.48 ± 0.80 mg/ml. Loading Fuc-S1 and Fuc-S2 into an intestinal Caco-2 system to test the associated reactions for 90 min revealed that the transportation and cellularabsorption rates of Fuc-S1 were 28.1% and 39.0%, respectively, whereas those of Fuc-S2 were 31% and 47.6%, respectively.
Relation: 42 (1)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39555
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