English  |  正體中文  |  简体中文  |  Items with full text/Total items : 26994/38795
Visitors : 2392263      Online Users : 69
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39478

Title: O-Carboxymethyl Chitosan/Fucoidan Nanoparticles Increase Cellular Curcumin Uptake
Authors: Yi-Cheng Huang
Tzu-Hung Kuo
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Cellular uptake
Curcumin
Chitosan
Fucoidan
Nanoparticle
Date: 2016-02
Issue Date: 2016-12-14T07:09:48Z
Publisher: Food Hydrocolloids
Abstract: Abstract: Curcumin (Cur) is a polyphenolic compound extracted from turmeric (Curcuma longa), and exhibits several biological activities like antitumor, antioxidant, inhibiting cardiovascular diseases and inducing apoptosis. However, because of extremely low solubility and sensitivity to pH values, Cur is easily degradable in the gastrointestinal tract, resulting in reduced bioavailability. In this study, we developed pH-sensitive O-carboxymethyl chitosan/fucoidan (O-CMC/F) nanoparticles (NPs) for Cur delivery, and evaluated their physicochemical properties and influence on cells. Our results suggest that O-CMC and F reacted with calcium ions to obtain ionic crosslinked NPs of 100–200 nm. At a weight ratio of O-CMC to F of 1:1, the O-CMC/F NPs exhibited pH-sensitive properties. The Cur-loaded O-CMC/F NPs was 270 nm and encapsulated 92.8% Cur. Encapsulated Cur was stable in a simulated limosis pH environment (pH 2.5). However, in a simulated intestinal pH environment (pH 7.4), Cur was released from the O-CMC/F NPs. Encapsulated Cur was associated with considerably lower cytotoxicity to mouse fibroblasts cells (L929) than free Cur. When we used Caco-2 cells as an in vitro model to evaluate cellular uptake, we observed that the Cur-loaded O-CMC/F NPs were internalized by the cells through energy-dependent endocytic pathways. In conclusion, pH-sensitive O-CMC/F NPs effectively increase the cellular uptake of Cur and can potentially be used as carriers in oral delivery systems.
Relation: 53
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39478
Appears in Collections:[食品科學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML100View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback