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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39426

Title: Composition characterization of Myctophids (Benthosema pterotum): antioxidation and safety evaluations for Myctophids protein hydrolysates
Authors: Chang-Jer Wu
Huey-Jine Chai
Yi-Lin Chan
Tsung-Lin Li
Yu-Chen Chen
Chwen-Herng Wu
Chyuan-Yuan Shiau
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Acute toxicity
Protein hydrolysate
Antioxidant activity
Ames test
Date: 2012-04
Issue Date: 2016-12-12T07:27:36Z
Publisher: Food Research International
Abstract: Abstract: Myctophids (Benthosema pterotum) were regarded as low-valued small deep-sea fishes. In this study, we however identified that B. pterotum contains high levels of proteins, free amino acids and some functional components (e.g. phospholipids, polyunsaturated fatty acids and minerals). Converting Myctophids from a low-valued fish to a high value-added protein source was thus attempted. The content of nitrogen compounds in the protein hydrolysates of B. pterotum (BPH) (by autolysis) accounted for 53.6% of peptides with Mw less than 1500 Da. BPH was also identified to possess high levels of reducing power (1.26 ± 0.04 abs), Fe2 +-chelating activity (95.73 ± 2.05%) and DPPH scavenging activity (94.01 ± 1.00%), which correspond to 100 ppm of ascorbic acid, 100 ppm of EDTA and 20 ppm of ascorbic acid, respectively. We further determined that the in vitro effective concentrations of BPH for eliminating 50% free radicals (EC50) on DPPH scavenging, Fe2 +-chelating, reducing power and superoxide oxide dismutase (SOD) activity are 5.66 μM, 3.74 μM, 12.27 μM and 56.37 μM, respectively. For food safety, BPH was evaluated by both Ames test (using Salmonella Typhimurium TA98) and acute toxicity in a rat model. No mutagenicity arose on the levels of BPH ranging from 625 to 5000 μg with/without metabolic activation. No treatment-related changes (e.g. general condition, body weight, ophthalmology, hematology, and histopathology) were found in rats. For the no-observed-adverse-effect-level (NOAEL), BPH was estimated to be no less than 15 g/kg in both male and female rats. The autolytic hydrolysates of BPH were concluded to be safe. Accordingly, BPH is an ideal raw material for the developments of protein-rich anti-oxidative healthy products.
Relation: 46(1)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39426
Appears in Collections:[食品科學系] 期刊論文

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