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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39424

Title: Chain elongation and cyclization in type III PKS DpgA
Authors: Chang-Jer Wu
Hai-Chen Wu
Yi-San Li
Yu-Chen Liu
Syue-Yi Lyu
Tsung-Lin Li
Contributors: 國立臺灣海洋大學:食品科學系
Date: 2012-04
Issue Date: 2016-12-12T07:20:55Z
Publisher: Chembiochem.
Abstract: Abstract: Chain elongation and cyclization of precursors of dihydroxyphenylacetyl-CoA (DPA-CoA) catalyzed by the bacterial type III polyketide synthase DpgA were studied. Two labile intermediates, di- and tri-ketidyl-CoA (DK- and TK-CoA), were proposed and chemically synthesized. In the presence of DpgABD, each of these with [(13)C(3)]malonyl-CoA (MA-CoA) was able to form partially (13)C-enriched DPA-CoA. By NMR and MS analysis, the distribution of (13)C atoms in the partially (13)C-enriched DPA-CoA shed light on how the polyketide chain elongates and cyclizes in the DpgA-catalyzed reaction. Polyketone intermediates elongate in a manner different from that which had been believed: two molecules of DK-CoA, or one DK-CoA plus one acetoacetyl-CoA (AA-CoA), but not two molecules of AA-CoA can form one molecule of DPA-CoA. As a result, polyketidyl-CoA serves as both the starter and extender, whereas polyketone-CoA without the terminal carboxyl group can only act as an extender. The terminal carboxyl group is crucial for the cyclization that likely takes place on CoA.
Relation: 13(6)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39424
Appears in Collections:[食品科學系] 期刊論文

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