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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39373

Title: Cells adhered and cultured on microcantilevers
Authors: Chih-Wei Wu;Sheng-Ping Chang;Chang-Jer Wu;Shih-Hao Huang
Contributors: 國立臺灣海洋大學:食品科學系
Date: 2013-01
Issue Date: 2016-12-07T08:17:57Z
Publisher: Microsystem Technologies
Abstract: Abstract: This paper shows a novel method to cultivate cells on a π-shape microcantilever inside a polydimethylsiloxane microfluidic system. Only one lithography step was needed to precisely align and pattern a poly(2-hydroxyethyl methacrylate) hydrogel microstructure, of size 200 × 200 μm, onto a silicon nitride microcantilever inside the PDMS microfluidic device. Gelatin was used as a sacrificial layer to resolve the issue of the microfluidic and hydrogel microstructure sticking together, successfully releasing the microcantilevers. BHK-21 cells were successfully laden and cultivated on the hydrogel microstructures of microcantilevers for 24 h. The optical system consisted of a He–Ne laser, a charge-coupled device camera, and a position-sensitive detector, which was used to measure the deflections of the microcantilevers due to the laden cells. The deflection increased continually during the cell-laden period. Meanwhile, the deflection increased with increasing cell concentration. By repeating the cell-laden and culture experiment three times, the magnitude and trend of deflection of microcantilevers were almost the same. It demonstrates that the microcantilever-based biochip has adequate stability and provides reliable measurement results for drug screening applications in the future.
Relation: 19(1)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39373
Appears in Collections:[食品科學系] 期刊論文

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