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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39370

Title: A cancer vaccine based on the marine antimicrobial peptide pardaxin (GE33) for control of bladder-associated tumors
Authors: Han-Ning Huang
Venugopal Rajanbabu
Chieh-Yu Pan
Yi-Lin Chan
Chang-Jer Wu
Jyh-Yih Chen
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Cytokines
Antimicrobial peptide
Bladder tumor
Cancer vaccine
Date: 2013-12
Issue Date: 2016-12-07T07:40:53Z
Publisher: Biomaterials
Abstract: Abstract: The marine antimicrobial peptide (AMP) GE33, also known as pardaxin, possesses antimicrobial and anticancer properties, and modulates host signaling. GE33 has cytotoxic effects on murine bladder carcinoma (MBT-2) cells. Here, we investigated the potential of GE33 combined with inactivated MBT-2 as a cancer vaccine. The presence of up to 12.5 μg of GE33 did not inhibit the proliferation or endogenous nitrous oxide (NO) levels of RAW264.7 cells. However, the secretion of MCP-1, IL-6, and IL-12 by RAW264.7 cells was affected by GE33. We proceeded to test the effectiveness of the vaccine by immunizing mice at 7, 14, and 21 days of age, and injecting live MBT-2 cells on the 28th day. Tumor growth by the 58th day was attenuated in mice treated with the vaccine, as compared to the control group. Induction of MBT-2 specific-tumor antigens was increased in mice immunized with our vaccine. Furthermore, activation of T-cell receptors, cytotoxic T-cells, and NK cells was enhanced, and these showed high specificity for targeting tumor cells. Finally, immunization controlled excess recruitment of monocytes, lymphocytes, T-helper cells, and NK cells, and decreased the expression of VEGF. This report provides empirical evidence that our GE33-based vaccine enhances antitumor immunity in mice.
Relation: 34(38)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39370
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