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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39345

Title: Improved elution of isoamylase adsorbed on raw starch and the preservation of purified enzyme
Authors: Long-Liu Lin;Tsuei-Yun Fang;Wen-Shen Chu;Wen-Hwei Hsu
Contributors: 國立臺灣海洋大學:食品科學系
Date: 1994-11
Issue Date: 2016-12-07T01:46:26Z
Publisher: Letters in Applied Microbiology
Abstract: Abstract: The isoamylase from Pseudomonas amyloderamosa can be recovered by adsorption—elution on raw starch. In this process, the elution of adsorbed isoamylase was significantly affected by elution design. When the raw starch-enzyme suspension was packed into a funnel-type glass filter instead of being stirred in a flask to elute the adsorbed isoamylase, the recovery was increased from 53.6% to about 81% and the concentration of isoamylase was also increased 42-fold in the eluted solution. The addition of 1% potassium sorbate into the eluted enzyme solution gave a retention of more than 80% of the recovered isoamylase activity in a 5-month storage at 4°C.
Relation: 19(5)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39345
Appears in Collections:[食品科學系] 期刊論文

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